Toxicity Study of Chlorzoxazone and Isosorbide Dinitrate using Chick Embryo

Number of potential drugs are underutilized due to a lack availability teratological data. Isosorbide Dinitrate is saviour drug in angina prophylaxis while chlorzoxazone skeletal muscle relaxant and there no adequate teratogenic study performed till date. This aims assess the effect these on vital organs using chick embryo model. White Leghorn’s (Gallus gallus domesticus) fertilised chicken eggs were acquired from shivneri agro hatcheries Nashik divided into five groups (n=10) as Control, nonteratogenic, teratogenic, chlorzoxazone, Dinitrate. The was injected via yolk inoculation after inoculation; re-incubated at 37.5-37.8°C 50-60% RH for 21 days. Then embryos harvested evaluated morphological histopathological changes. gross macroscopic examination treated chicks normal. development found shunted group. Microscopic abrasions group myocardial congestion, hemorrhage, hydropic degeneration, dislocation nucleus, splitting cells, infiltration cells all three doses. No response observed hence be safe. Teratogenic Chlorzoxazone isosorbide dinitrate provided notable details. safe developmental phase, While highest dose toxic so, it inadvisable its utilization pregnancy.